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One of the frequent uses of the technique is to date organic remains from archaeological sites.Plants fix atmospheric carbon during photosynthesis, so the level of C level for the calculation can either be estimated, or else directly compared with known year-by-year data from tree-ring data (dendrochronology) up to 10,000 years ago (using overlapping data from live and dead trees in a given area), or else from cave deposits (speleothems), back to about 45,000 years before the present.This resemblance is used in chemical and biological research, in a technique called carbon labeling: carbon-14 atoms can be used to replace nonradioactive carbon, in order to trace chemical and biochemical reactions involving carbon atoms from any given organic compound.

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Its existence had been suggested by Franz Kurie in 1934. The primary natural source of carbon-14 on Earth is cosmic ray action on nitrogen in the atmosphere, and it is therefore a cosmogenic nuclide.This is small compared to the doses from potassium-40 (0.39 m Sv/year) and radon (variable).

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